Identification of NADPH oxidase in the vasculature
NADPH oxidase(s), major source(s) of superoxide in a variety of tissues, was first described in neutrophils as involved in the respiratory burst. The following publications from our group are among the first in the field to describe and characterize Nox in a non-phagocytic cell. These findings constitute the basis of what is now known to be an important proximal signaling pathway and a major culprit in the development of myriad diseases.
Al Ghouleh I, Meijles DN, Mutchler S, Zhang Q, Sahoo S, Gorelova A, Henrich Amaral J, Rodríguez AI, Mamonova T, Song GJ, Bisello A, Friedman PA, Cifuentes-Pagano ME, Pagano PJ. Binding of EBP50 to Nox organizing subunit p47phox is pivotal to cellular reactive species generation and altered vascular phenotype. Proc Natl Acad Sci U S A. 2016; 113 (36):E5308-17. PubMed PMID: 27540115, PMCID: PMC5018796
Pagano PJ, Ito Y, Tornheim K, Gallop PM, Tauber AI, Cohen RA. An NADPH oxidase superoxide-generating system in the rabbit aorta. Am J Physiol. 1995; 268: H2274-80.PubMed PMID: 7611477.
Pagano PJ, Clark JK, Cifuentes-Pagano ME, Clark SM, Callis GM, Quinn MT. Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: enhancement by angiotensin II. Proc Natl Acad Sci U S A. 1997, 94(26):14483-8.PubMed PMID: 9405639, PMCID: PMC25029
Pagano PJ, Chanock SJ, Siwik DA, Colucci WS, Clark JK. Angiotensin II induces p67phox mRNA expression and NADPH oxidase superoxide generation in rabbit aortic adventitial fibroblasts. Hypertension. 1998; 32(2):331-7. PubMed PMID: 9719063